SibTech, Inc.

Optimization of Proteins for Multiple Applications

SibTech provides Custom Protein Optimization Service, which is rapid, flexible, and comprehensive. It includes the following stages:

1. Your protein is re-engineered for expression with N- or C-terminal Cys-tag.
2. Cys-tagged protein is expressed, refolded, purified, and tested for functional activities in vitro.
3. A payload you need is site-specifically conjugated to Cys-tagged protein. We are confident in our ability to conjugate virtually any payload. Our menu of standard payloads includes:
   1. Fluorescent dyes for in vivo and in vitro targeted imaging
   2. Radionuclide chelator for SPECT targeted imaging
   3. Radionuclide chelator for PET targeted imaging
   4. Lipids for formulation into liposomes or other lipid-based formulations
   5. PEG for extending clearance time
   6. Other proteins, such as those used in tissue scaffold, implant, or biosensors engineering
4. Conjugates are purified and tested for functional activities in vitro.
5. If necessary, adapter-based conjugates are made for self-assembly with your Cys-tagged protein.

In our experience, a new conjugate can be made and validated in vitro in 2 to 4 months. At that point you would have a powerful R&D tool, for example, for targeted imaging. The new conjugate is then evaluated in vivo, for a rational decision regarding development of your protein for new clinical application(s).